Genetic Testing And Identification Of Dna Extraction

Introduction. Extracting DNA from available cells is a widely used practice with applications such as forensics, genetic testing and victim identification to name a few. DNA extractions have been widely practiced for decades. This report simply summarises our results comparing two methods. The tests compared the effectiveness of the often cited ‘salting out method’ based on Sunnucks and Hales’ (1996) method of DNA extraction from aphids, with a ‘science-at-home’ style method of DNA extraction according to the NHGRI’s video â€Å"how to extract DNA from strawberries† (2010) This made use of household detergent as a surfactant to allow the DNA out of the cell and its contents. This method is cheaper to run, not involving costlier laboratory chemicals like Proteinase K. (Ciulla, Sklar and Hauser, 1988, page 488) Each requires high precision to differentiate between samples from different individuals, and it is ideal to produce accurate results from a small sample. The sample must consist only of DNA for gel electrophoresis to give proper results, containing no protein or lipid impurities. The samples must be pure to be comparable, something that the YouTube method cannot offer with reliability due to not using Proteinase K, which was applied early in the salting out method to denature proteins for later removal. The YouTube method is predicted to obtain DNA, but not with the same consistency or accuracy as the salting out method, due to the lack of time andShow MoreRelatedAnnotated Bibliography On Dna Fingerprinting1019 Words   |  5 PagesDNA fingerprinting is a scientific technology involving the extraction, replication and arrangement of strands of an organism’s DNA. This results in the formation of a genetically distinctive fingerprint that is unique to the organism which the DNA sample was originally extracted from. Because of the specificity of a DNA fingerprint, the application of this technology can have a substantial influence on many aspects of society. Accessibility to a DNA database allows for higher efficiency in forensicRead MoreDna Barcoding Essay766 Words   |  4 Pagespast decade, DNA barcoding has emerged as an advanced molecular diagnostic tool for species identification. DNA barcoding relies on a biological barcode or uniform region of DNA to probe organisms. T he term ‘DNA barcode’ is applied to a standardized short sequence of DNA. It can be used as a unique identification marker to recover and characterize organisms or species. It identifies unknown samples by matching a specific genetic marker to a reference sequence library[1-3]. An ideal DNA barcode hasRead MoreWhat is DNA Profiling?1659 Words   |  7 PagesAnthropology Forensic DNA Analysis What is DNA Profiling? There are many different terms used in conjunction to DNA Profiling; DNA testing, DNA typing and genetic fingerprinting. This is a technique which is used by Forensic Scientists by means of assistance in the identification of individuals by their DNA profiles. A DNA profile is an encrypted set of numbers w0hich reflects an individual’s DNA makeup. This encryption can also be used when identifying a person. DNA profiling is NOT howeverRead MoreGenetics Module 7 Lab 2 Essay1692 Words   |  7 Pagesï » ¿******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted:   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   a. Genetic testing   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   b. Body Identification   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   c. Forensic analysis 3. Name the piece of equipment used to obtain the cellsRead MoreThe Theory Of The Germ Theory1122 Words   |  5 Pagesinject a health animal with a small dose of the disease they won’t contract the disease. But Koch was able to further his research on the disease and its life cycle. It was the work of Pasture and Koch that opened the door for other researchers to identification of disease-causing and lifesaving treatments. b) For- The Germ theory ï  ¶ The Washing hand theory showed that midwives cleaned their hand more often than doctors which then later showed there were more deaths with doctors than midwivesRead MoreImpact Of Dna Fingerprinting3430 Words   |  14 PagesDNA profiling technologies have had a considerable impact on how forensic science and criminal investigation have been understood, carried out, and regulated in the last 25 years. Current methods of forensic DNA profiling (known also as DNA fingerprinting and DNA typing), based on Polymerase Chain Reaction (PCR) amplifications of a varying number of Short Tandem Repeat (STR) loci found at different locations on the human genome, are regularly described as constituting the â€Å"gold standard for identification†Read MoreDn The Hereditary Material1685 Words   |  7 PagesDeoxyribonucleic acid (DNA) is the hereditary material in humans. It is contained in the nucleus of nearly every cell in the human body and is unique to all individuals except in c ases of identical twins. Thus, DNA can be used to identify suspects in forensic casework. DNA can be left behind at forensic crime scenes in a variety of ways. Common sources of human DNA at crime scenes include: saliva, blood, semen, skin, and hair follicles. Saliva samples can provide high-quality and high-quantity DNA, but they doRead MoreBenefits of Immunotherapy from Advances in Immunology and Recombinant Dna Technology3196 Words   |  13 PagesBenefits of Immunotherapy from advances in immunology and recombinant DNA technology. Immunotherapy is a form of medical treatment intended to stimulate or restore the ability of the immune system to fight infection and disease. This can be by inducing, enhancing, or suppressing an immune response. Immunotherapies designed to elicit or amplify an immune response are classified as activation immunotherapies, while those that reduce or suppress immune response are suppression immunotherapies. ActiveRead MoreThe Role of Dna Technology in Crime Investigation5441 Words   |  22 PagesDNA profiling DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals on the basis of their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a persons DNA makeup, which can also be used as the persons identifier. DNA profiling should not be confused with full genome sequencing. It is used in, for example, parental testing and rape investigationRead MoreDna Testing And Law Enforcement Officers Essay1825 Words   |  8 PagesDNA forensics is a division of forensic science that focuses on the use of genetic material in criminal investigation to answer questions pertaining to legal situations, including criminal and civil cases. Through DNA testing, law enforcement officers are able to identify human remains or the individual responsible for a crime. DNA testing is a highly advanced scientific process that involves replicating the human DNA sequenc e to create a genetic map of an individual. Because of its reliability,

Nelly Dean, the Narrator of Emily Brontes Wuthering Heights

Wuthering Heights: Nelly the Narratornbsp;nbsp;nbsp;nbsp;nbsp; nbsp; Emily Bronte wrote the book Wuthering Heights from the narrative point of view of Nelly, a servant who lived most of her life with Catherine. Many have questioned why Bronte would do so.nbsp; Why did she not choose someone with more knowledge? Why did she not choose a major character like Heathcliff or Catherine?nbsp; The choice to make Nelly the narrator is what makes the book so great. She is one who qualifies most to be the narrator. nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; This book is very much about love and hate, and Nelly is the one who is totally un-opinionated about the characters.nbsp; She was like a mother figure,†¦show more content†¦81)nbsp; Hareton probably does not remember her, which shows the lack of appreciation everyone had for her.nbsp; No one praised her like nbsp;Heathcliff and Edgar praised Catherine, but no one hated her like Hindly hated nbsp;Heathcliff.nbsp; In fact, she was the middle man that made the transition fromnbsp; Wuthering Heights to Thrushcross Grange so smooth.nbsp; If she had not been the narrator we would not know what went on at Thrushcross Grange.nbsp; Nelly manages to know what went on at Wuthering Heights because of her good relationship with everyone.nbsp; So, even though Nelly was not present there, she still had the capabilities to know what was going on.nbsp; nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; Though the story does not contain a specified â€Å"mother figure†, Nellys role implies just that.nbsp; Oddly, every time a lad was born, the mother died during the birth. This led to Nellys mother like role.nbsp; She did not play her mother like role merely with Hareton and Cathy, (the two lads whos mothers died during labor) but she also played the role with Heathcliff and Catherine. ***IS CATHY A LAD?***nbsp; When Heathcliff retaliated against Hindly and decided not to eat due to his pride, Nelly brought him someShow MoreRelated The Narrative Structure of Wuthering Heights and Heart of Darkness1152 Words   |  5 PagesThe Narrative Structure of Wuthering Heights and Heart of Darkness      Ã‚  Ã‚   Wuthering Heights by Emily Bronte was first published in 1847, during the Victorian Era. Heart of Darkness by Joseph Conrad was first published as a complete novel in 1902, beginning what is referred to as the Modernist Era in literature. Each of these compelling stories is narrated by an uninvolved character who is quoting a story told to them by a character who actually participated in the story being told. There areRead MoreEssay on Emily Brontes Wuthering Heights1127 Words   |  5 PagesEmily Brontes Wuthering Heights       Often in literature, the fictional written word mimics or mirrors the non-fictional actions of the time. These reflections may be social, historical, biographical, or a combination of these. Through setting, characters, and story line, an author can recreate in linear form on paper some of the abstract concepts and ideas from the world s/he is living in. In the case of Emily Bronte, her novel Wuthering Heights very closely mirrors her own life and the livesRead MoreThe Depth of Emily Brontes Wuthering Heights884 Words   |  4 PagesWuthering Heights was written by Emily Bronte’. It would be the least to say her imagination was quite impressive. Through imagination as a child, Bronte’ and her sisters would write children stories, which inspired some popularly known novels. Wuthering Heights contains crossing genres, changing settings, multiple narrators, and unreliable narrators. George R. R. Martin wrote the book Game of Thrones, which is one of the modern day no vels that contain several of Emily Bronte’s writing techniquesRead MoreEmily Bronte s Wuthering Heights1283 Words   |  6 PagesEmily Brontà «Ã¢â‚¬â„¢s Wuthering Heights is told from a complicated point of view. The narrator of the past is Nelly Dean, while the present time narrator is Mr. Lockwood. Set as a story within a story, Nelly tells Lockwood an eyewitness account of her dealings with the Earnshaws and the Lintons, while Lockwood is the outsider who records in his diary Nelly’s stories of Wuthering Heights and Thrushcross Grange. From the novel’s beginning, Lockwood proves himself to be objective, while Nelly is subjectiveRead MoreWuthering Heights by Charlotte Bronte1143 Words   |  5 Pagesa devilish, preternatural passion that tamer beings can scarcely recognize as love.† (Duclaux) Wuthering Heights by Emily Brontà « is considered a masterpiece today, however when was first published, it received negative criticism for its passionate nature. Critics have studied the novel from every analytical angle, yet it remains one of the most haunting love stories of all time. â€Å"Wuthering Heights is not a comfortable book; it invites admiration rather than love.† (Stoneman) The novel containsRead MoreWuthering Heights, by Emily Brontà «1865 Words   |  8 Pagesdevilish, preternatural passion that tamer beings can scarcely recognize as love.† (Duclaux) Wuthering Heights by Emily Brontà « is considered a masterpiece today, however when it was first published, it received negative criticism for its passionate nature. Critics have studied the novel from every analytical angle, yet it remains one of the most haunting love stories of all time. â€Å"Wuthering Heights is not a comfortable book; it invites admiration rather than love,† (Stoneman 1). The novel containsRead MoreWuthering Heights By Emily Bronte1198 Words   |  5 PagesIntroduction Brontà «Ã¢â‚¬â„¢s novel depicts the addictive yet destructive relationship between orphaned Heathcliff and Catherine. Wuthering Heights repeats cycles of relationships from the 1st generation of the Linton’s, Earnshaw’s, and Heathcliff’s transferred on to their children, the 2nd generation- Linton, Hareton, and the 2nd Catherine. Amongst this cycle, amorous and vengeful relationships are amid the family. However, the 2nd generation is able to accomplish the goal set by Heathcliff and the firstRead MoreEssay about A Comparison of Wuthering Heights and Heart of Darkness831 Words   |  4 Pages A Comparison of Wuthering Heights and Heart of Darkness nbsp; Emily Brontes Wuthering Heights and Joseph Conrads Heart of Darkness are two similar stories in the effect that they both have dual narrators and that the narrators of both are manipulated to tell stories of similar morals.nbsp; They differ, however, in the narrative frames, points of view, and some personality traits of the narrators. nbsp; The dual narrator arrangement of Wuthering Heights begins with Mr. Lockwood, theRead MoreEmily Brontes Wuthering Heights1590 Words   |  7 PagesEmily Bronte created a book called Wuthering Heights that was published in 1847. The book has been rejected multiple times by the Victorian readers because of its disturbing, unexplained vision of anarchy and decay (Knoepflmacher). I chose the book Wuthering Heights because it has an interesting name. I never thought the book was narrated by two people and that it had a dramatic romance to it. Also I have notice that there is a large amount of hate towards the character Heathcliff due to his actionsRead MoreEmily Brontà «Ã¢â‚¬â„¢s Personal Influence on Wuthering Heights1820 Words   |  8 Pagesthe case in Wuthering Heights by Emily Brontà «. Although the novel is in itself fictional, Brontà « invites readers into her private life by the way in which she writes her novel. Literary elements are often taken into consideration when determ ining the value of a literary work. However, they offer more than just layers of complexity to a work. Brontà « uses countless metaphors to portray relevance to her own life. The ongoing comparison between the characters in Wuthering Heights and Brontà «Ã¢â‚¬â„¢s own life only

Evaluation And Human Services Management †Myassignmenthelp.Com

Question: Discuss About The Evaluation And Human Services Management? Answer: Introducation Evaluation is a methodical and purposeful judgment of a phenomenon, programme or policy so that one can infer some logical data from the outcome that has been recorded. Some of the basic concepts that are associated with evaluation are methods for decision making, ways of measuring and testing a subject, collection of relevant information about the subject, processing the gathered information into a logical path, concluding from the information gathered (Boulmetis Dutwin, 2014). Evaluation is the process by which one can understand the extent to which the set objective has been met. Evaluation is a dynamic process that has a continuous growth (Vedung, 2017). Human Service Human services can be defined as the process of meeting the objectives of basic human demands. This can be obtained through various means like heterogeneous yet intertwined understanding of the human needs, developing a focused procedure to comprehend with the issues that are related to human wants, finding out and researching on the solutions to these problems (Sundel Sundel, 2017). Human services are basically an approach towards elevating the quality of life of service populations. Human services offers agenda or amenities so that people are able to get basic health facilities, wellbeing, and also keep a track of the marginalized, financially challenged and sick people who have no other fall back option (Monette, Sullivan DeJong, 2013). As the human service is a profession which has a lot of abstract factors associated with the job, it is almost non quantifiable in the sense that one cannot quantify charity or the feeling of charity with which people opt for human service. There are several organizations that offer human services as non-profit seeking organizations. Most of the times it is a challenge for such organizations to evaluate and measure the impact of their work. Every person has the right to see what has been the impact or the outcome of their hard work; in case of human services the determination of the effect of service is difficult to measure. However, there are several measuring instruments used by the organizations to understand their work. Human services are a genre which has a long term objectives and the short term goals of the organizations hardly show any impact or positive measures. Constructive outcomes of the services that are provided by the people of human service usually are related to the changes in the society, hence there are no specific linear or suitable explanations of the results. Human services change is hard to evaluate. Most of the time the results that are quantifiable are indirect in nature that is they do not show the impact of change in the society they are just a measured quantity of money or commodity that may bring some changes. For example: when an organization arranges a fundraiser for some cause, there are some expenses that are required to arrange the event these expenses are barred by the organization and then there is the income that has been gathered from the event. In this case, the fund that has been raised by the organization is quantifiable through accounting methods, but the chang es that this endeavor is set to bring for the society is non quantifiable because the status of living of a person cannot change overnight by a fundraising event (Morrow Jr et al., 2015). One of the tension points of the evaluation of human services is the trust factor which is an abstract emotion that cannot be measured in terms of quantity or quality. Trust is based on mutual understanding and hence it is difficult to quantify. Another pressure point of human services is that the perception of positive and negative in the minds of people are subjective. For example, if one thinks that the fundraiser was a success because it has gathered a lot of money, it is possible that another may think that the money raised is not enough. The contemporary discussions and debates that are raised in this topic are finding a logical, rational method of evaluation of service, there should be segregation of evaluation a simple accounting is just one phase of the human service evaluation process there should be Impact evaluations in a logical way. It should concentrate on questions of causality. What was the impact of the event? Was the impact at par with the anticipated result? If no then what went wrong? How could things change in the long run? If it has a positive impact then, what has helped the event and what activities or characteristics of the program created the impact? Did the program have any unintended consequences, positive or negative? (Mohammed Mariani, 2014). Reference List: Boulmetis, J., Dutwin, P. (2014).The ABCs of evaluation: Timeless techniques for program and project managers(Vol. 56). John Wiley Sons. Mohammed, D., Mariani, R. (2014). An Evaluation of the Cybersecurity Policies for the United States Health Human Services Department: Criteria, Regulations, and Improvements.International Journal of Business and Social Research,4(4), 1-7. Monette, D. R., Sullivan, T. J., DeJong, C. R. (2013).Applied social research: A tool for the human services. Cengage Learning. Morrow Jr, J. R., Mood, D., Disch, J., Kang, M. (2015).Measurement and Evaluation in Human Performance, 5E. Human Kinetics. Sundel, M., Sundel, S. S. (2017).Behavior change in the human services: Behavioral and cognitive principles and applications. Sage Publications. Vedung, E. (2017).Public policy and program evaluation. Routledge.

Roman Holiday free essay sample

Deep mahogany wood glides under my feet; the floorboard creeks halfway between the closet and the TV. I walked into the hotel room. I threw my red cross-body bag onto the middle bed and claimed it as mine. I continued to make a beeline straight for the window. After a long day of travel I needed to see the city. I opened the shutters and inhaled. This was it. I was in Rome. At the end of sixth grade I had to register for a foreign language for the seventh grade. Having taken one trimester of culture connections I could count to ten in five different languages but beyond that I had no concept of the languages. One afternoon when I was talking to all my friends about what classes they would take, and Kathryn brought up something about Latin class. Her brother, then a freshman in high school, knew several people who took Latin and got to go to Rome. We will write a custom essay sample on Roman Holiday or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Suddenly I knew what language I was going to take. The images in my head immediately placed me in The Lizzie McGuire movie. I pictured myself standing in line on cobblestone pavement waiting to enter a small stooped building housing an artisan shop. While I somehow knew I wouldn’t be discovered as a pop-star look alike in Rome, I had a hope that something amazing would happen there. Almost immediately I started saving my money. I made a deal with my parents that if I could save one thousand dollars, they would pay for the rest of the trip. I got a â€Å"job† babysitting for my sister’s friend’s family once a week after school. In the entire span of my middle school years I don’t think I spent more than fifty dollars total on the things I wanted. Every single penny I had went towards Rome, this was what I wanted and I was going to make it happen. After two years of working through Latin in middle school, I entered high school and advanced to Latin II. Highlights of that year included square dancing to a song about the endings of certain words and standing on my chair shouting, â€Å"Curse you, third declension!† with 20 other kids doing just the same. When I walked into my Latin III class sophomore year I had no idea how quickly my dream of Rome would become a reality. My teacher was a twenty something who had spent time studying in Italy when he was in college, so I knew he would show us all the best spots on the trip.. Again the year involved plenty of things that could only happen in Latin. Latin was simply the best part of my day, and it furthered my drive to go to Italy. Finally the parent-student meetings began, and all the kids from the high schools met one another. We were able to see who else we would be spending a week with halfway across the world. I had set my goals. I knew I had wanted this. I was going to Italy and that was that. As I boarded the airplane and caught my first glimpse at the cabins made for trans-Atlantic flights I knew that if I put my mind to anything I could make it happen. I have gravel that got caught in my shoe from when I climbed to the top of Mount Vesuvius, I translated the inscription on the Arch of Septimius Severus, and followed in the footsteps of Emperors along the Sacra Via. Just knowing how hard I worked to get there, made everything that I did while I was there that much more impactful. I learned so many more things on that trip than I can ever mention in one essay, and I’ll take those things with me the rest of my life.