Genetic Testing And Identification Of Dna Extraction

Introduction. Extracting DNA from available cells is a widely used practice with applications such as forensics, genetic testing and victim identification to name a few. DNA extractions have been widely practiced for decades. This report simply summarises our results comparing two methods. The tests compared the effectiveness of the often cited ‘salting out method’ based on Sunnucks and Hales’ (1996) method of DNA extraction from aphids, with a ‘science-at-home’ style method of DNA extraction according to the NHGRI’s video â€Å"how to extract DNA from strawberries† (2010) This made use of household detergent as a surfactant to allow the DNA out of the cell and its contents. This method is cheaper to run, not involving costlier laboratory chemicals like Proteinase K. (Ciulla, Sklar and Hauser, 1988, page 488) Each requires high precision to differentiate between samples from different individuals, and it is ideal to produce accurate results from a small sample. The sample must consist only of DNA for gel electrophoresis to give proper results, containing no protein or lipid impurities. The samples must be pure to be comparable, something that the YouTube method cannot offer with reliability due to not using Proteinase K, which was applied early in the salting out method to denature proteins for later removal. The YouTube method is predicted to obtain DNA, but not with the same consistency or accuracy as the salting out method, due to the lack of time andShow MoreRelatedAnnotated Bibliography On Dna Fingerprinting1019 Words   |  5 PagesDNA fingerprinting is a scientific technology involving the extraction, replication and arrangement of strands of an organism’s DNA. This results in the formation of a genetically distinctive fingerprint that is unique to the organism which the DNA sample was originally extracted from. 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